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| 产品名称： | p425 GPD |
|---|---|
| 商品货号： | TS140731 |
| Designations： | p425 GPD |
| Depositors： | M Funk |
| Biosafety Level： | 1 |
| Host： | Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Intact vector size: 7.76
Type of vector: plasmid Cloning sites: XhoI SalI HindIII PstI SmaI BamHI SpeI Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI Other unique sites: SacI Construction: pRS425 Host range: Saccharomyces cerevisiae, Candida robusta, Escherichia coli Features (with orientation and position when available): marker(s): LEU2, ->, 200-2446 terminator: CYC1, ->, 3128-3388 MCS: XhoI...XbaI, ->, 3388-3452 promoter for expression: GPD, <-, 3452-4132 marker(s): ampR, <-, 5284-6214 replicon: 2 micron, ->, 6313-7653 Vector: p425 GPD (plasmid)
Promoters: Promoter for expression GPD
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| Applications： | YE-type (episomal) shuttle vector YX-type (expression) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.9, 3.2, 0.7; EcoRI--5.5, 2.3; BamHI--7.8. High copy number shuttle expression vector. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504 |
| Related Products： | Component of: ATCC 87669 |
| Shipped： | frozen |