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| 产品名称： | pMOB45 |
|---|---|
| 商品货号： | TS140753 |
| Designations： | pMOB45 |
| Depositors： | D Vapnek |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 10.5000000000000000 Vector: pMOB45 (plasmid) Promoters: Promoter cml Construction: R1, pBR322 Marker(s):cmlR,tetR Construct size (kb): 10.5 Features: marker(s): cmlR, tetR promoter: cml, tet replicon: R1(ts) |
| Applications： | expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--8.0; ClaI--8.0; EcoRI--8.0; HindIII--8.0. Restriction digests of the clone give the following sizes (kb): BamHI--8.0; ClaI--8.0; EcoRI--8.0; HindIII--8.0. An expression vector with copy number amplifiable by heat induction without protein synthesis inhibition. |
| Media： | ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Bittner M, Vapnek D. Versatile cloning vectors derived from the runaway-replication plasmid pKN402. Gene 15: 319-329, 1981. PubMed: 6277736 |
| Shipped： | freeze-dried |