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| 产品名称： | pMMB278 |
|---|---|
| 商品货号： | TS140768 |
| Designations： | pMMB278 |
| Depositors： | VM Morales |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.9899997711181640 Vector: pMMB278 (plasmid) Promoters: Promoter tac Construction: pMMB190, pMMB311 Marker(s):ampR Construct size (kb): 8.989999771118164 Features: insert detection: lacZ marker(s): ampR promoter: tac replicon: oriV, oriT (IncQ) repressor gene: lacIq transcription terminator: rrnB |
| Applications： | expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--9.0; EcoRV--5.4, 3.7; SspI--5.0, 1.7, 1.5, 0.9; EcoRI/MluI--8.2, 0.8. Contains sequences encoding lacIq, a tac promoter, and lacZalpha to allow direct screening by inactivation of alpha-complementation. Constructed by replacing the MluI/EcoRI fragment of pMMB190 (taclacUV5 promoter) with the MluI/EcoRI fragment of pMMB311 (tac promoter). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Morales VM, et al. A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants. Gene 97: 39-47, 1991. PubMed: 1847347 |
| Shipped： | freeze-dried |