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| 产品名称： | lambda20-20 |
|---|---|
| 商品货号： | TS140770 |
| Designations： | lambda20-20 |
| GenBank Number： | X12660 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 44.0 |
| Insert： | DNA: genomic Insert lengths(kb): 14.0 Tissue: follicular lymphoma (library ATCC 77181) Gene product: immunoglobulin switch mu, rearranged IGHMS* |
| Insert Size (kb)： | 14.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--20.0, 15.0, 9.0; EcoRI--20.0, 15.0, 9.0; EcoRI/PvuII--17.0, 10.0, 4.6, 4.3, 3.9, 1.8, 0.6; HindIII--40.0, 4.6; PstI--17.0, 7.0, 4.6, 2.8, 2.5, 2.0, 1.8, 1.5, 1.2, 1.15, 0.85, 0.55, 0.3; SalI--20.0, 9.0, 5.0 (triplet). The sequenced portion also contains a PvuII, a HindIII and an EcoRI site. This clone was isolated from an EMBL4 library, grown on E. coli LE392 and characterized at the Cytogenetics and Cancer Genetics laboratory of Memorial Sloan Kettering Cancer Center, New York. Date of isolation - Sept.-Oct. 1986. A 2.54 kb PstI fragment from the insert has been sequenced (X12660) and contains a rearranged Smu-Cmu region. The sequenced fragment contains 26 direct repeats 10-44 bp long, 51 inverted repeats, and more than 20 putative Ig recombinational signals. |
| References： | Sabyasachi Ganguly, personal communication |