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| 产品名称： | pcD-2A-E3 pcD-IL-BSF1/TCGFII/MCGFII |
|---|---|
| 商品货号： | TS140887 |
| Designations： | pcD-2A-E3 pcD-IL-BSF1/TCGFII/MCGFII |
| GenBank Number： | M13238 |
| Species： | Mus musculus, mouse |
| Depositors： | DNAX Research Institute, SC Macevicz, DNAX Research Institute |
| Applications： | in suitable host, produces protein B-cell stimulatory factor 1 |
| Vector： | Construct size (kb): 3.6 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pcDV1 Intact vector size: 3.000 Type of vector: plasmid Cloning sites: SalI ClaI HindIII EcoRI KpnI Polylinker sites: Construction: pBR322, SV40 Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): enhancer: none marker(s): ampR promoter: none replicon: pMB1 terminator: SV40 late Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: mouse Gene symbol: bcsf1* Gene name: B-cell stimulatory factor 1 Contains complete coding sequence?: Y Tissue: T-cell cell line Cl.Ly1+2-1/9 (ATCC CRL-8179) Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 0.6 Cross references: DNA Seq. Acc.: M13238 Nucleotides 1-585 of the insert correspond to nucleotides 1-585 of M13238. Insert lengths(kb): 0.60 Tissue: T-cell cell line Cl.Ly1+2-1/9 (ATCC CRL-8179) Gene product: B-cell stimulatory factor 1 bcsf1* Target Gene: B-cell stimulatory factor 1 |
| Insert Size (kb)： | 0.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Nucleotides 1-585 of the insert correspond to nucleotides 1-585 of M13238. The insert contains the following unique restriction sites: SacI; AhaIII; PstI(although there are two sites in the vector); ClaI; XmnI; ScaI. Restriction digests of the clone give the following sizes (kb): BamHI--2.8, 0.8; EcoRI--3.6; HindIII--3.6. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Lee F, et al. Isolation and characterization of a mouse interleukin cDNa clone that expresses B-cell stimulatory factor 1 activities and T-cell and mast-cell-stimulating activities. Proc. Natl. Acad. Sci. USA 83: 2061-2065, 1986. PubMed: 3083412 Lee F, et al. cDNA clones coding for human protein exxhibiting a broad cellular activity spectrum (human interleukin-4). US Patent 5,552,304 dated Sep 3 1996 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |