产品名称: | BL29-16 |
---|---|
商品货号: | TS140907 |
Designations: | BL29-16 |
GenBank Number: | T25299 T25300 |
Species: | Homo sapiens, human |
Depositors: | R Sanders |
Vector: | Construct size (kb): 3.460000038146973 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
Insert: | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: D1S1730E Gene name: DNA Segment, single copy, expressed Contains complete coding sequence?: U Chromosome: 1 Tissue: BL29 cell line Disease: Burkitts lymphoma Type of DNA: cDNA Insert end: Modification: EcoRI linker Insert end: Modification: EcoRI linker Insert size (kb): 0.5 Cross references: DNA Seq. Acc.: T25299; EST: EST016; Primer: T7 DNA Seq. Acc.: T25300; EST: EST016R; Primer: T3 Insert lengths(kb): 0.5 Tissue: BL29 cell line Burkitts lymphoma Disease: Burkitts lymphoma Gene product: DNA Segment, single copy, expressed D1S1730E |
Insert Size (kb): | 0.5 |
Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information: | Distributed: freeze-dried |
Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.3, 0.2; BamHI--3.45; HindIII--3.45; KpnI--3.45; PstI--3.45. |