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微信小章| 产品名称： | pHU10 plasmid in E. coli |
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| 商品货号： | TS141005 |
| Designations： | pHU10 plasmid in E. coli |
| Depositors： | TP Dryja |
| Biosafety Level： | 1 |
| Host： | Escherichia coli K12 |
| Vector Information： | Total size of construct (kb): 7.2 Marker: ampR Enzyme to excise insert: HindIII Vector: pBR322 Vector type: plasmid Vector size (kb): 4.363 Markers: ampR Vector ends: HindIII |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--4.4, 2.8; PstI--7.2. A multiple site polymorphism also detected with XmnI and EcoRI, with a common band of 6.0 and alleles of 8.5 and 10 kb. An unpublished RFLP is detected by this probe when genomic DNA is digested with EcoRI. The published RFLP is revealed with XmnI. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Dryja TP, et al. Chromosome 13 restriction fragment length polymorphisms. Hum. Genet. 65: 320-324, 1984. PubMed: 6319270 Dryja TP, Morton CC. Mapping of seven polymorphic loci on human chromosome 13 by in situ hybridization. Hum. Genet. 71: 192-195, 1985. PubMed: 4065891 Misra BC, Srivatsan ES. Localization of HeLa cell tumor-suppressor gene to the long arm of chromosome II. Am. J. Hum. Genet. 45: 565-577, 1989. PubMed: 2577469 Lundberg C, et al. Loss of heterozygosity in human ductal breast tumors indicates a recessive mutation on chromosome 13. Proc. Natl. Acad. Sci. USA 84: 2372-2376, 1987. PubMed: 3031679 Leppert M, et al. A primary genetic map of chromosome 13q. Am. J. Hum. Genet. 39: 425-437, 1986. PubMed: 3464200 Thaddeus P Dryja, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Freeze-dried E. coli containing the plasmid |