产品名称: | alpha12-Q229L pcDNAI |
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商品货号: | TS141076 |
Designations: | alpha12-Q229L pcDNAI |
Species: | Mus musculus, mouse |
Depositors: | HR Bourne |
Applications: | contains sequence G protein, alpha subunit |
Vector: | Construct size (kb): 6.0 |
Insert: | DNA: cDNA Insert lengths(kb): 2.0 Gene product: G protein, alpha subunit Target Gene: G protein, alpha subunit |
Insert Size (kb): | 2.0 |
Media: | ATCC® Medium 1951: LB medium (ATCC medium 1065) with 50 mcg/ml ampicillin and 15 mcg/ml tetracycline |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information: | Distributed: freeze-dried |
Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI--6.0; HindIII/XbaI--4.0, 2.0; BglII--6.0. Contains G protein alpha 12 with a Q229->L activating mutation. Expresses protein in mammalian cells. T7 promoter is the closest to 5 end. |
Classification: | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
References: | Voyno-Yasenetskaya T, et al. G alpha 13 stimulates Na-H exchange. J. Biol. Chem. 269: 4721-4724, 1994. PubMed: 8106440 |