| 产品名称: | alpha12-Q229L pcDNAI |
|---|---|
| 商品货号: | TS141076 |
| Designations: | alpha12-Q229L pcDNAI |
| Species: | Mus musculus, mouse |
| Depositors: | HR Bourne |
| Applications: | contains sequence G protein, alpha subunit |
| Vector: | Construct size (kb): 6.0 |
| Insert: | DNA: cDNA Insert lengths(kb): 2.0 Gene product: G protein, alpha subunit Target Gene: G protein, alpha subunit |
| Insert Size (kb): | 2.0 |
| Media: | ATCC® Medium 1951: LB medium (ATCC medium 1065) with 50 mcg/ml ampicillin and 15 mcg/ml tetracycline |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: freeze-dried |
| Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI--6.0; HindIII/XbaI--4.0, 2.0; BglII--6.0. Contains G protein alpha 12 with a Q229->L activating mutation. Expresses protein in mammalian cells. T7 promoter is the closest to 5 end. |
| Classification: | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References: | Voyno-Yasenetskaya T, et al. G alpha 13 stimulates Na-H exchange. J. Biol. Chem. 269: 4721-4724, 1994. PubMed: 8106440 |