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| 产品名称： | YIp5-32cat |
|---|---|
| 商品货号： | TS141101 |
| Designations： | YIp5-32cat |
| Depositors： | H Feldmann |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.6100001335144040 Vector: YIp5-32cat (plasmid) Promoters: Promoter none Construction: YIp5, YRp7-32cat (ATCC 37624) Marker(s):URA3,ampR Construct size (kb): 6.610000133514404 Features: insert detection: CAT marker(s): ampR, URA3 promoter: none replicon: pMB1 |
| Applications： | YI-type (integrating) shuttle vector integrating vector promoter-cloning vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.8, 0.64, 0.58; PstI--3.85, 3.20; PvuII--3.7, 3.25. The depositor recommends using YRp7-17camp (ATCC 37630) as the reference for CAT expression and using the CAT assay methodology of Neumann, et al. One of a series (ATCC 37624 through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast. A 5195 bp BamHI/HindIII fragment of YIp5 was ligated to a 1415 bp HindIII/BamHI fragment of YRp7-32cat (ATCC 37624). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mannhaupt G, et al. A series of shuttle vectors using chloramphenicol acetyltransferase as a reporter enzyme in yeast. Gene 67: 287-294, 1988. PubMed: 3049257 |
| Shipped： | freeze-dried |