微信小陈
微信小章
产品简介
购买须知
| 产品名称： | YCplac111 |
|---|---|
| 商品货号： | TS141232 |
| Designations： | YCplac111 |
| Depositors： | RD Gietz |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.112
Vector: YCplac111 (plasmid)
Promoters: Promoter lac
Construction: pUC19
Marker(s): LEU2,ampR
Features: insert detection: lacZ marker(s): LEU2 marker(s): ampR promoter: lac replicon: ARS1 replicon: pMB1 MCS: HindIII...EcoRI centromere: CEN4 |
| Applications： | YC-type (centromeric) shuttle vector vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII--6.5, EcoRV--6.5, BglI--4.7, 1.7. One of 9 shuttle vectors (ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1). The EcoRI and KpnI restriction sites from the LEU2 gene of S. cerevisiae were removed by using oligo-directed mutagenesis. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Gietz RD, Sugino A. New yeast Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534, 1988. PubMed: 3073106 |
| Related Products： | component of:ATCC 87623 component of:ATCC 87638 |
| Shipped： | frozen |
| Shipping Information： | Frozen E. coli containing the plasmid |