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| 产品名称： | pVNTR-B |
|---|---|
| 商品货号： | TS141242 |
| Designations： | pVNTR-B |
| Species： | Homo sapiens, human |
| Applications： | A 0.6 kb TaqI fragment has been used to detect the VNTR polymorphism. |
| Vector： | Construct size (kb): 6.900000095367432 |
| Insert： | DNA: genomic Insert lengths(kb): 4.199999809265137 Gene product: active BCR-related gene ABR |
| Insert Size (kb)： | 4.200 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--4.4, 2.6; EcoRI--7.0; HindIII--7.0; BamHI--7.0; PstI--6.6, 0.4. The insert is the 4.2 kb HindIII/EcoRI fragment containing VTR B and exons 4 and 5. The repeat elements are in the HindIII/XhoI fragment of about 1.8 kb. The exons are close to the EcoRI end, with exon 5 contained in a SstI/EcoRI fragment of about 0.3 kb. BamHI, BglI, BstEII, EcoRI, HindIII and SstI cut outside the VNTR, generating one fragment per allele, but the sizes are difficult to resolve. A 0.6 kb TaqI fragment has been used to detect the VNTR polymorphism. TaqI cuts within the VNTR generating 1 to 4 different sized alleles per individual. HinfI also cuts within the VNTR. |
| References： | Heisterkamp N, et al. ABR, an active BCR-related gene. Nucleic Acids Res. 17: 8821-8831, 1989. PubMed: 2587217 DiSilvestre D, et al. A hypervariable RFLP within the ABR gene. Nucleic Acids Res. 18: 5924, 1990. PubMed: 1977147 Deborah Disilvestre, personal communication |