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| 产品名称： | pXC33b |
|---|---|
| 商品货号： | TS141271 |
| Designations： | pXC33b |
| Depositors： | TA Patterson |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.7760000228881840 Vector: pXC33b (plasmid) Promoters: Promoter for expression lambda PL Construction: pXC24, oligo cassette Marker(s):ampR Construct size (kb): 5.776000022888184 Features: initiation codon: ATG marker(s): ampR marker(s): tetR promoter for expression: lambda PL replicon: ROP copy number control replicon: pMB1 restriction site: 3 sites HindIII...BamHI restriction site: 5 PacI site ribosome-binding site: synthetic Shine-Dalgarno spacer sequence: ATTAATTA transcription terminator: partially deleted lambda terminator translational enhancer: T7 gene 10 coding sequence: 14-3-3 |
| Applications： | expression vector in another host, produces protein vector useful for cloning PCR products |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.8; EcoRI--5.8; HindIII--5.8. The vector contains a 0.7 kb bovine cDNA that can be excised using the 5 and 3 cloning sites and be replaced by a gene of interest. The bovine insert may be used as a positive control for expression. One of 12 expression vectors (ATCC 86990-87001) designed to maximize expression from the lambda PL promoter and support cloning of PCR products. The vectors differ in cloning sites and in translational enhancer and initiation sequences. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Cheng X, Patterson TA. Construction and use of lambda PL promoter vectors for direct cloning and high level expression of PCR amplified DNA coding sequences. Nucleic Acids Res. 20: 4591-4598, 1992. PubMed: 1408761 |
| Shipped： | freeze-dried |
| Shipping Information： | Shipped: Freeze dried Escherichia coli TAP308 containing the plasmid. |