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微信小章| 产品名称： | Clostridioides difficile (Prevot) Lawson et al. |
|---|---|
| 商品货号： | TS141290 |
| Deposited As： | Clostridium difficile (Hall and OToole) Prevot |
| Application： | Enteric Research |
| Isolation： | Clinical specimen |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Preceptrol®： | no |
| Genotype： | Toxinotype: IIIc; |
| Type Strain： | no |
| Comments： | Presence of tcdA and tcdB genes confirmed by PCR. Ref   Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303Confirmed by CDC: NAP1, Toxinotype III, binary toxin positive Binary Toxin gene cdtB was amplified by PCR Toxinotype IIIc Ribotype 027 |
| Medium： | ATCC® Medium 2107: Modified Reinforced Clostridial |
| Growth Conditions： | Temperature: 37°C Atmosphere: Anaerobic |
| Name of Depositor： | R Clark |
| References： | Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303 Stubbs, S et al. Production of actin-specific ADP-ribosyltransferase (binary toxin) by strains of Clostridium difficile. FEMS Microbiol. Lett. 186(2): 307-312, 2000. PubMed: 10802189 Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986 |