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| 产品名称： | pNMU-1 |
|---|---|
| 商品货号： | TS141344 |
| Designations： | pNMU-1 |
| Species： | Rattus norvegicus, rat |
| Depositors： | M Barbacid |
| Vector： | Construct size (kb): 11.39999961853027 |
| Insert： | DNA: genomic Insert lengths(kb): 6.800000190734863 Tissue: transformed 3T3 cell line Gene product: Harvey rat sarcoma viral (v-Ha-ras) oncogene homolog(Harvey rat sarcoma virus oncogene) HRAS |
| Insert Size (kb)： | 6.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Constructed from DNA from NIH 3T3 cell transformed by rat mammary tumor induced by nitroso-methyl urea. Restriction digests of the clone give the following sizes (kb): BamHI--6.8, 4.5; HindIII--5.6, 3.15, 2.5; EcoRV--5.8, 5.2; EcoRI--11.5. Insert contains NaeI, PvuII (3), SacI (3), HindIII (2), MstI, BstEII (2), HpaI (2), and EcoRV sites. Four exons demonstrated. G to A transition in codon 12 leads to activation of this p21. |
| References： | Sukumar S, et al. Induction of mammary carcinomas in rats by nitroso-methylurea involves malignant activation of H-ras-1 locus by single point mutations. Nature 306: 658-661, 1983. PubMed: 6318112 |