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| 产品名称： | Op-30 |
|---|---|
| 商品货号： | TS141387 |
| Designations： | Op-30 |
| GenBank Number： | X13694 |
| Species： | Homo sapiens, human |
| Applications： | The cell-binding sequence and a potential N-linked glycosylation site are conserved in both human and rat. |
| Vector： | Construct size (kb): 4.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.399999976158142 Tissue: osteosarcoma Gene product: secreted phosphoprotein 1 (includes osteopontin)( 44kDa bone phosphoprotein, Secreted phosphoprotein-1 (osteopontin, bone sialoprotein), bone sialoprotein, osteopontin, early T lymphocyte activation, osteopontin) OPN Alleles: A1, A1, A2, B1, A2, B1, B2, B2 |
| Insert Size (kb)： | 1.400 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Insert contains the following sites separated by (bp): (BamHI/BglII)- 272- AhaIII- 480- PvuII- 129- EcoRI- 209- XmnI- 116- HindIII- 61- SspI- 157- (BglII/BamHI). (BamHI and BglII sites destroyed in cloning.). Restriction digests of the clone give the following sizes (kb): XbaI/PstI--2.75, 1.38; XbaI--4.0; PstI--4.0; EcoRI--3.5, 0.56; HindIII--3.6, 0.34. The 5 EcoRI fragment of the clone detects genomic EcoRI fragments of 6.8 kb, 2.1 kb, and 0.9 kb in the human; 5.5 kb in the hamster; and 6.6 kb in the mouse. It also detects a human genomic BamHI fragment of 25 kb and KpnI fragment of 11.9 kb. This probe is a full-length cDNA. The human and rat polypeptide precursors are 65% identical. The cell-binding sequence and a potential N-linked glycosylation site are conserved in both human and rat. Enzyme(s) not detecting polymorphism: EcoRI, HindIII, BamHI, XbaI, sacI, TaqI, PvuII, PstI, EcoRV, KpnI. |
| References： | Kiefer MC, et al. The cDNA and derived amino acid sequence for human osteopontin. Nucleic Acids Res. 17: 3306, 1989. PubMed: 2726470 |