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| 产品名称： | pCG19 |
|---|---|
| 商品货号： | TS141407 |
| Designations： | pCG19 |
| Depositors： | M Keller |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.0479998588562000 Vector: pCG19 (plasmid) Promoters: Promoter lambda PR Construction: lambda pR promoter and cI857 repressor cloned into pCRII, ligated to pSC101/par and tetR from pLG339 Marker(s):tetR Construct size (kb): 5.0479998588562 Features: marker(s): tetR other: par locus promoter: lambda PR replicon: pSC101 repressor gene: cI857 |
| Applications： | expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): XbaI - 5.0; XhoI - 5.0; PstI - 5.0. Low copy number expression vector for overexpression of cloned bacterial* genes in E. coli from the lambda pR promoter, induced by temperature or pH shift, (the latter according to literature only). |
| Media： | ATCC® Medium 6025: LB Medium with 12 mcg/ml tetracycline |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Gotting C, et al. Versitile low-copy number plasmids for temperature-inducible overexpression of bacterial genes in Escherichia coli. BioTechniques 24: 362-366, 1998. PubMed: 9526640 |
| Shipped： | frozen |