产品名称: | Human adenovirus 2 |
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商品货号: | TS141411 |
Classification: | Adenoviridae, Mastadenovirus, Human adenovirus C |
Agent: | Human adenovirus 2 |
Strain: | ATCC-2011-7 |
Applications: | Respiratory research |
Biosafety Level: | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format: | frozen |
Storage Conditions: | -70°C or colder |
Comments: | This virus was isolated in cell culture at ATCC. |
Effect on Host: | CPE, refractile cells with typical lattice formation |
Recommended Host: | A549 (ATCC CCL-185) |
Growth Conditions: | Temperature: 37°C Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted in VGM to provide an optimal MOI (e.g. MOI 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 4-6 days |
Name of Depositor: | ATCC |
Source: | Clinical patient positive for Adenovirus, Virginia, July 2011 |