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| 产品名称： | Influenza A virus (H1N1) |
|---|---|
| 商品货号： | TS141421 |
| Classification： | Orthomyxoviridae, Influenzavirus A |
| Deposited As： | Influenza A virus |
| Agent： | Influenza A virus (H1N1) |
| Strain： | A/PR/8/34 |
| Applications： | Influenza Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | VR-1469® is a TC-adapted item derived from virus passaged in eggs (ATCC® VR-95™). |
| Effect on Host： | CPE, rounding, degeneration, sloughing |
| Recommended Host： | MDCK cellsxa0(ATCC® CCL-34™) |
| Growth Conditions： | Temperature: 35°C Recommendations for Infection: For best results, infection should be performed on an 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer two times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 2-4 days at 35°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | ATCC®, M Coleman, Centers for Disease Control and Prevention |
| Special Collection： | NCRR Contract |
| Source： | Patient in Puerto Rico, 1934 |
| References： | Francis T. Immunological relationships of strains of filterable virus recovered from cases of human influenza. Proc. Soc. Exp. Biol. Med. 32: 1172-1175, 1935. |