微信小陈
微信小章
产品简介
购买须知
| 产品名称： | PEmbl 600 pEmbl 600 q |
|---|---|
| 商品货号： | TS141466 |
| Designations： | PEmbl 600 pEmbl 600 q |
| Species： | Homo sapiens, human |
| Applications： | DNA from secondary transfectants was used to isolate the clone. |
| Vector： | Construct size (kb): 4.400000095367432 |
| Insert： | DNA: genomic Insert lengths(kb): 0.6000000238418579 Tissue: NK14 cell line DNA transferred into NIH 3T3 cells Gene product: mel transforming oncogene (derived from cell line NK14)- RAB8 homologue(ras-associated protein RAB8) MEL |
| Insert Size (kb)： | 0.600 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): AvaI--3.85, 0.69; ClaI--4.4; EcoRI--4.4; SalI--4.4; HindIII--4.4. Contains Sau3A (225), AccI (300), HinfI (345), DdeI (365), and SacII (540) sites (approximate distances in bp from 5 end). Suggest using the AvaI/Sau3A fragment as there are 50 bp of Alu sequences 3 to Sau3A site. NIH 3T3 cells were transformed with DNA from the melanoma NK14 cell line. DNA from secondary transfectants was used to isolate the clone. |
| References： | Padua RA, et al. A novel transforming gene in a human malignant melanoma cell line. Nature 311: 671-673, 1984. PubMed: 6090953 Spurr NK, et al. Chromosomal assignment of c-mel, a human transforming oncogene, to chromosome 19 (p13.2-q13.2). Somatic Cell Mol. Genet. 12: 637-640, 1986. PubMed: 3466361 Rose. Ann Padua, personal communication |