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| 产品名称： | YCp50-poly |
|---|---|
| 商品货号： | TS141618 |
| Designations： | YCp50-poly |
| Depositors： | DJ Stillman |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.0560002326965330 Vector: YCp50-poly (plasmid) Construction: YCp50, pUC19 polylinker Marker(s):URA3,ampR Construct size (kb): 7.056000232696533 Features: marker(s): URA3 marker(s): ampR replicon: ARS1 replicon: pMB1 MCS: EcoRI...HindIII centromere: CEN4 |
| Applications： | YC-type (centromeric) shuttle vector vector for constructing genomic libraries |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--7.2; SmaI--5.1, 2.2; PstI--3.3, 1.8, 1.1, 0.9; PvuI--7.2. YC-type shuttle vector. YCp50-poly was constructed in two steps: (1) YCp50 (ATCC 37419) was cleaved with XhoI and Asp718, blunted and ligated. (2) This construct was then cleaved with EcoRI and SalI, and the EcoRI-PvuII fragment of pUC19 was inserted. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Rose MD, et al. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. Gene 60: 237-243, 1987. PubMed: 3327750 Wang H, Stillman DJ. Transcriptional repression in Saccharomyces cerevisiae by a SIN3-lexA fusion protein. Mol. Cell. Biol. 13: 1805-1814, 1993. PubMed: 8441414 |
| Related Products： | component of:ATCC 87635 |
| Shipped： | freeze-dried |
| Shipping Information： | xa0Freeze dried E. coli containing the plasmid. |