微信小陈
微信小章| 产品名称： | pPNP1 |
|---|---|
| 商品货号： | TS141636 |
| Designations： | pPNP1 |
| GenBank Number： | K02574 |
| Species： | Homo sapiens, human |
| Depositors： | DW Martin |
| Vector： | Construct size (kb): 6.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.417999982833862 Tissue: HeLa cell line Gene product: nucleoside phosphorylase NP |
| Insert Size (kb)： | 1.418 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--4.4, 0.7, 0.58; BglII--6.0; EcoRV--4.4, 1.55; KpnI--6.0; BamHI--4.8, 1.2. Although there is a AATAAA sequence in the insert, is is 208 bp from the 3 end of the insert which does not contain poly(A). Thus this is probably not the authentic polyadenylation signal. Each end of the insert contains 20-22 dC/dG bp from the cloning procedure. The single long open reading frame encodes 289 amino acids, corresponding to a molecular weight of 32.1 kDa. |
| References： | Osborne WR, Miller AD. Design of vectors for efficient expression of human purine nucleoside phosphorylase in skin fibroblasts from enzyme-deficient humans. Proc. Natl. Acad. Sci. USA 85: 6851-6855, 1988. PubMed: 3137573 Williams SR, et al. Human purine nucleoside phosphorylase cDNA sequence and genomic clone characterization. Nucleic Acids Res. 12: 5779-5787, 1984. PubMed: 6087295 Goddard JM, et al. Cloning of human purine-nucleoside phosphorylase cDNA sequences by complementation in Escherichia coli. Proc. Natl. Acad. Sci. USA 80: 4281-4285, 1983. PubMed: 6410388 Williams SR, et al. A human purine nucleoside phosphorylase deficiency caused by a single base change. J. Biol. Chem. 262: 2332-2338, 1987. PubMed: 3029074 |