产品名称: | c8145 8145 |
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商品货号: | TS141647 |
Designations: | c8145 8145 |
Species: | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
Depositors: | MV Olson, L Riles |
Applications: | probe for fluorescence in situ hybridization (FISH) (unlabelled) DNA Segment |
Vector: | Construct size (kb): 47.90000152587891 |
Insert: | DNA: genomic Insert lengths(kb): 41.5 Gene product: DNA Segment Target Gene: DNA Segment |
Insert Size (kb): | 41.5 |
Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
Related Products: | component of:ATCC 77284 |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Comments: | Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 4.097, 2.447, 0.524, 8.171, 11.171, 1.894, 1.547, 0.711, 1.041, 2.141, 3.293, 2.710, 1.213, 0.643. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. A centromere-distal probe. The end of the insert proximal to the centromere is 295.5 kb from CEN16. Overlaps clones with ATCC number(s): 70184, 70190, 70957, 71032. NOTE: Because of the large number of simultaneous deposits from Dr. Olson, the ATCC has not verified that the characteristics of the material supplied here are as published. The ATCC encourages users of this material to communicate their results to us. |
Classification: | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
References: | Guacci V, et al. Centomere position in budding yeast: evidence for anaphase A. Mol. Biol. Cell 8: 957-972, 1997. PubMed: 9201708 |