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| 产品名称： | Vaccinia virus |
|---|---|
| 商品货号： | TS141654 |
| Classification： | Poxviridae, Orthopoxvirus |
| Deposited As： | Vaccinia virus, Orthopoxvirus |
| Agent： | Vaccinia virus |
| Strain： | IHD-W Dts28 |
| Applications： | The mutation was assigned by complementation analysis to vaccinia map location A24, the 132-kDa viral RNA polymerase subunit. |
| Biosafety Level： | 2 BSL 2Vaccination recommended for lab staff mipulating vaccinia, monkeypox, or cowpox viruses
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | This conditional lethal mutant, originally designated plaque isolate no. 3679, was isolated from the IHD-W strain of Vaccinia by S. Dales. The IHD-W strain was derived from an IHD-J strain of Vaccinia. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | Selected after mutagenesis with nitrosoguanidine. The non-permissive incubation temperature is 39.5 C. This mutant was assigned a Dales EM categories G and P, characterized by incomplete membranes with spicules, immature normal particles and mature particles with cores that are predominantly lucent at the center. The mutation was assigned by complementation analysis to vaccinia map location A24, the 132-kDa viral RNA polymerase subunit. |
| Effect on Host： | CPE: enlargement, plaques with rounding and eventual sloughing Cytopathic effects in cell culture include cell rounding and death, with eventual sloughing |
| Growth Medium： | ATCC® Medium 30-2003: Eagles Minimum Essential Medium (EMEM) Fetal Bovine Serum |
| Recommended Host： | Production Host: BSC-40 (ATCC CRL-2761) Recommended Host: BSC-40 (ATCC CRL-2761) Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2 |
| Growth Conditions： | 1-3 days at 31 C with 5% CO2 |
| Name of Depositor： | S Dales, R. Condit |
| References： | Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807 Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570 |