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| 产品名称： | pNK2811 |
|---|---|
| 商品货号： | TS141665 |
| Designations： | pNK2811 |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | N Kleckner |
| Applications： | produces protein transposase, Tn10 |
| Vector： | Construct size (kb): 9.199999809265137 |
| Insert： | DNA: genomic Insert information: Insert size (kb): 7.2 Genexa0: transposase, Tn10 DNAxa0: genomic Sourcexa0: Escherichia coli Sequencedxa0? Partial (see reference) Insert lengths(kb): 7.199999809265137 Gene product: transposase, Tn10 Target Gene: transposase, Tn10 |
| Insert Size (kb)： | 7.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Freeze dried E. coli containing the plasmid |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--9.2; HindIII--6.6, 2.5; PstI--5.2, 2.95, 0.98; XbaI--6.8, 2.1. Construct permitting translational fusions of kan to target genes. Kanamycin resistance is expressed after transpositions in the correct orientation and reading frame into an expressed target gene. Contains a 2.2 kb mini-Tn10 cassette conferring erythromycin resistance and a promoterless kan gene (kan) gene, bounded by inverted repeats of the outermost 70 bp of IS10R and embedded (as an EcoRI/HindIII fragment) in hisG and hisD. Also contains the wild type transposase gene regulated by the Ptac promoter, inducible by IPTG, oriented opposite to kan, and separated from kan by 4 tandem repeats of the rrnB terminator. The order of the major features in the insert is: XbaI - HindIII - transposase - Ptac - rrnB terminators - HindIII - kan - erm - EcoRI - XbaI. |
| Classification： | Enterobacteriaceae, Escherichia |
| References： | Kleckner N, et al. Uses of transposons with emphasis on Tn10. Methods Enzymol. 204: 139-180, 1991. PubMed: 1658561 |