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| 产品名称： | pcDL-SRalphahCD40L |
|---|---|
| 商品货号： | TS141705 |
| Designations： | pcDL-SRalphahCD40L |
| GenBank Number： | L07414 |
| Species： | Homo sapiens, human |
| Applications： | in another host, produces protein CD40 antigen ligand |
| Vector： | Construct size (kb): 3.859999895095825 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pcDL-SRalpha296 Intact vector size: 3.700 Type of vector: plasmid Vector end: BamHI Vector end: BamHI Cloning sites: BamHI Polylinker sites: Construction: pcDV1, HTLV-1 LTR Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): ampR promoter: SV40/HTLV-1 LTR replicon: pMB1, SV40 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CD40LG Other gene symbols: CD40L Gene name: CD40 antigen ligand Contains complete coding sequence?: Y Chromosome: X; Localization: X q26 Tissue: CD4-positive T-cells Type of DNA: cDNA Insert end: BamHI Insert end: BamHI Insert size (kb): 0.9 Cross references: DNA Seq. Acc.: L07414 Insert lengths(kb): 0.8999999761581421 Tissue: CD4-positive T-cells Gene product: CD40 antigen ligand CD40L Target Gene: CD40 antigen ligand |
| Insert Size (kb)： | 0.900 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--3.4, 1.5; NcoI--3.5, 1.4; PvuII--4.9; SalI--2.4, 2.5; BamHI--3.9, 1.0. The insert contains the following restriction sites (approximate kb from the 5 end): HindIII--0.58; NcoI--0.78; PvuII--0.52. Contains the complete coding sequence (nt 40-825 of the sequence record). COS-7 cells transfected with this construct demonstrate good surface expression of the protein. The vector is modified from pcD, with a promoter constructed from the SV40 early promoter and the R segment and U5 sequence of the long terminal repeat of human T-cell leukemia virus type 1 (HTLV-1 LTR). Subcloned from pBS-hCD40L-6A9 (ATCC 79812/3). The 5 BamHI site is derived from the vector and the 3 end is the naturally occurring BamHI site (nt 929 of the published sequence). |
| References： | Gauchat JF, et al. Human CD40-ligand: molecular cloning, cellular distribution and regulation of expression by factors controlling IgE production. FEBS Lett. 315: 259-266, 1993. PubMed: 7678552 Gauchat JF, et al. Induction of human IgE synthesis in B cells by mast cells and basophils. Nature 365: 340-343, 1993. PubMed: 7690905 Jean-Francois Gauchat, personal communication |