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| 产品名称： | pEM303 pHUMDUT2 |
|---|---|
| 商品货号： | TS141770 |
| Designations： | pEM303 pHUMDUT2 |
| GenBank Number： | M89913 |
| Species： | Homo sapiens, human |
| Depositors： | EM McIntosh |
| Applications： | in another host, produces protein dUTP pyrophosphatase |
| Vector： | Construct size (kb): 3.700000047683716 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pTZ19R Intact vector size: 2.900 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Construction: pUC19, Escherichia coli lacZalpha, pBR322, f1 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, T7 replicon: pMB1, f1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: DUT* Genomic copy number: multiple Gene name: dUTP pyrophosphatase (dUTPase) Contains complete coding sequence?: Y Chromosome: 15; Localization: 15, cR Type of DNA: cDNA Insert end: Modification: EcoRI adapters Insert end: Modification: EcoRI adapters Insert size (kb): 0.8 Cross references: DNA Seq. Acc.: M89913 Insert lengths(kb): 0.800000011920929 Gene product: dUTP pyrophosphatase DUT* Target Gene: dUTP pyrophosphatase |
| Insert Size (kb)： | 0.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--3.7; SmaI--3.7; SphI--3.7; EcoRI--2.9, 0.8; PstI--3.7. The insert contains the following restriction sites (nt from the 5 end): SphI--43; SmaI--91, XmnI--653. Contains the complete coding sequence, and corresponds to nt 43-837 of the database record. The sense strand is packaged. pEM303 (TS141770/7) and pEM304 (ATCC 79038/9) differ only in the orientation of the insert. |
| References： | McIntosh EM, et al. Human dUTP pyrophosphatase: cDNA sequence and potential biological importance of the enzyme. Proc. Natl. Acad. Sci. USA 89: 8020-8024, 1992. PubMed: 1325640 Evan M McIntosh, personal communication |