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| 产品名称： | Galpha i2 alpha i2-WT (mouse), pBN31 |
|---|---|
| 商品货号： | TS141775 |
| Designations： | Galpha i2 alpha i2-WT (mouse), pBN31 |
| GenBank Number： | M13963 |
| Species： | Mus musculus, mouse |
| Depositors： | P Herzmark, HR Bourne |
| Applications： | in another host, produces protein guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 |
| Vector： | Construct size (kb): 5.360000133514404 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.365000009536743 Gene product: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 Gnai2 Target Gene: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 |
| Insert Size (kb)： | 1.365 |
| Media： | ATCC® Medium 1890: LB medium (ATCC medium 1065) with 12 mcg/ml tetracycline and 100 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.6, 0.8; BglI--3.1, 1.8, 0.55; BglII--5.35; EcoRI--4.0, 1.4, HindIII--5.35. The insert contains the following restrictions sites (approximate kb from the 5 end): BamHI--0.73; BglI--0.08; BglII--1.05; BstXI--0.26; EcoRI--1.34; PstI--0.37, 0.43; PvuII--0.41; SacI--0.94; SphI--0.82. Deposited with the understanding that the DNAs not be used in human experiments. Restriction site(s) at the 3 end of the insert for linearizing the construct: EcoRV, XhoI. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Wong YH, et al. Mutant alpha subunits of Gi2 inhibit cyclic AMP accumulation. Nature 351: 63-65, 1991. PubMed: 1851251 Paul Herzmark, personal communication |