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微信小章| 产品名称： | pJW101 |
|---|---|
| 商品货号： | TS141890 |
| Designations： | pJW101 |
| GenBank Number： | J00153 |
| Species： | Homo sapiens, human |
| Depositors： | BG Forget |
| Vector： | Construct size (kb): 6.349999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.6000000238418579 Gene product: hemoglobin, alpha 1( alpha 1 globin, globin, alpha 1) HBA1 |
| Insert Size (kb)： | 0.600 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--similar to uncut; HindIII--5.8, 0.65; BamHI--6.4; AvaI--4.7, 1.65; SmaI--uncut. The actual cDNA insert is about 600 bp. It cannot be excised as such because of AT tailing, but can be excised as a 1.6 kb MboII fragment containing 845 bp of pMB9. HindIII and MboII patterns of ATCC preparation match that of Forgets isolate. HindIII site 275 bp 3 to AT/exon 1 joint (in exon 2); 0.7 kb HindIII fragment contains 3 end of gene; 5.2 kb fragment contains 5 end; TaqI site 130 bp 5 to HindIII site. The 5 end at the insert coincides exactly with the ATG initiation site, 275 bp from the internal HindIII site. It lacks all the 5 untranslated sequences and the NcoI site bridging the initiation site. |
| References： | Wainscoat JS, et al. Association of two DNA polymorphisms in the alpha globin gene cluster. Am. J. Hum. Genet. 35: 1086-1089, 1983. PubMed: 6316779 Wilson JT, et al. Insertion of synthetic copies of human globin genes into bacterial plasmids. Nucleic Acids Res. 5: 563-581, 1978. PubMed: 345245 Wilson JT, et al. Nucleotide sequence of the coding portion of human alpha globin messenger RNA. J. Biol. Chem. 255: 2807-2815, 1980. PubMed: 6244294 Bernard G Forget, personal communication |