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pVEX2211 [EKA605]

货号 TS142029
中文名称 null
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产品名称: pVEX2211 EKA605
商品货号: TS142029
Designations: pVEX2211 EKA605
Depositors: DH Figurski
Biosafety Level: 1
Host:
Distribution host: Escherichia coli AS11
Vector Information:
Size (kb): 2.5999999046325680
DESCRIPTION OF VECTOR:
Intact vector size: 2.600
Type of vector: plasmid
Cloning sites:
Polylinker sites: EcoRI SalI BamHI BglII BssHII PvuI KpnI PstI PvuII ClaI NruI
SacI XhoI BglI HindIII
Construction: pVEX2111
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): cmLR
replicon: R6K
Vector: pVEX2211 (plasmid)
Construction: pVEX2111
Marker(s):cmlR
Construct size (kb): 2.599999904632568
Features: marker(s): cmlR
replicon: R6K
Applications:
host modification
vector for other uses
vector permitting positive selection for integration
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--2.75; HindIII--2.05, 0.65; PvuII--1.5, 1.2.
To be used with pVEX1211 or pVEX1212 (ATCC 77420, 77421).
The vector is used to clone a sequence flanking the target to be deleted. The vector will be integrated by homologous recombination at the cloned sequence when chloramphenicol selection is imposed in a host not expressing pir protein.
The deletion derivative retains one loxP site and the cmlR marker. The circularized, deleted sequence is lost from cells not expressing repA or pir.
In the presence of Cre recombinase (from E. coli EKA133 ATCC 47071), double cointegrates pVEX1211(pVEX1212) and pVEX2211(pVEX2212) provide two loxP sites for site-specific recombination that deletes the intervening target sequence.
Component of a vector-mediated excision system for generating in vivo deletions of large bacterial genomes, self-transmissible plasmids or temperate E. coli bacteriophages.
Differs from pVEX2212 (ATCC 77423) only in the orientation of the polylinker.
The order of the major features in the plasmid is: R6K ori - loxP - cmlR - NotI - HindIII/MCS/EcoRI - NotI - HindIII.
Media: ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions:
Temperature: 37.0°C
References:

Ayres EK, et al. Precise deletions in large bacterial genomes by vector-mediated excision (VEX). The trfA gene of promiscuous plasmid RK2 is essential for replication in several gram-negative hosts. J. Mol. Biol. 230: 174-185, 1993. PubMed: 8450534

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