产品名称: | phg22 |
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商品货号: | TS142082 |
Designations: | phg22 |
GenBank Number: | X52955 |
Species: | Homo sapiens, human |
Depositors: | EE Strehler |
Vector: | Construct size (kb): 5.0 |
Insert: | DNA: genomic Insert lengths(kb): 2.299999952316284 Tissue: leukocyte Gene product: calmodulin 2 pseudogene 2(calmodulin II pseudogene 2) CALM2P2 |
Insert Size (kb): | 2.300 |
Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information: | Distributed: freeze-dried |
Comments: | Restriction digests of the clone give the following sizes (kb): BamHI--2.7, 2.3; BglI--4.0, 1.1; EcoRI--3.6, 1.3; HindIII--4.4, 0.35 (doublet); PstI--3.3, 1.2, 0.6. The insert contains the following restriction sites (nt position according to the published sequence, with the RsaI site at nt 1 approximately 70 bp from the 5 BamHI site): RsaI--1, 1326, 1357, 1733, 1776, 1786, 1809, 1823; HindIII--285, 646; PstI--516, 1756; EcoRI--938. Contains the complete "coding" sequence which demonstrates high homology to calmodulin II-type cDNA (nt 905-1349 of the published sequence). Contains a target site duplication of 17 bp characteristic of retropseudogenes, and 3 copies of a tandemly arranged 34 bp direct repeat in the upstream region. |
References: | Koller M, et al. Characterization of two novel human retropseudogenes related to the calmodulin-encoding gene CaMII. Gene 97: 245-251, 1991. PubMed: 1999288 |