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| 产品名称： | pE8UCAT pE8UC, pEU-CAT |
|---|---|
| 商品货号： | TS142099 |
| Designations： | pE8UCAT pE8UC, pEU-CAT |
| Depositors： | G Piaggio |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.0000000000000000 Vector: pE8UCAT (plasmid) Promoters: Promoter none Construction: pEMBL8-CAT, pUMSSB1 Marker(s):ampR Construct size (kb): 7.0 Features: insert detection: CAT marker(s): ampR promoter: none replicon: pMB1 terminator: UMS enhancer: none |
| Applications： | promoter-cloning vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.6; EcoRI--5.4, 1.1, 0.4; HindIII--6.6. The presence of the terminator upstream of the cloning sites of the polylinker prevents transcription that starts at cryptic promoters in the vector sequence. This is a promoter cloning vector suitable for studying transcriptional activity of very weakly active promoter fragments. This was constructed by inserting a 1.025 kb SalI fragment from pUMSSB1 containing the strong transcription terminator from the mouse c-mos gene (UMS = upstream mouse sequence) into the EcoRI site of pEMBL8-CAT using EcoRI linkers. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Piaggio G, DeSimone V. A new expression vector to study weak promoters. Focus 12: 83-84, 1990. |
| Shipped： | freeze-dried |