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| 产品名称： | pGT4.5A |
|---|---|
| 商品货号： | TS142198 |
| Designations： | pGT4.5A |
| Depositors： | AL Joyner |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 9.5000000000000000 Vector: pGT4.5A (plasmid) Construction: En-2, lacZ, neo, pUC18 Marker(s):ampR Construct size (kb): 9.5 Features: insert detection: lacZ marker(s): ampR replicon: pMB1 terminator: SV40 polyadenylation |
| Applications： | contains sequence engrailed-2 reporter construct shuttle vector vector permitting construction of fusion proteins |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.8, 3.3, 1.8, 0.45, 0.2; HindIII--9.5; XbaI--4.6, 3.1, 1.0, 0.8. Gene trap vector designed to identify and mutate endogenous mammalian genes by integration and production of a fusion protein with the lacZ reporter gene. Can also be used to monitor endogenous gene expression. Identified cDNA sequences spanning the lacZ splice junction can be cloned using the published sequence of the En-2 splice acceptor and the rapid amplification of cDNA ends (RACE) protocol. Derived from pGT4.5 by replacing the En-2 polyadenylation signal with an SV40 polyadenylation signal. The order of the major features in this plasmid is: pUC18 - HindIII - En-2 intron - splice acceptor - En-2 homeobox exon - lacZ - SV40 polyadenylation - human beta-actin promoter - neoR - SV40 polyadenylation. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Skarnes WC, et al. A gene trap approach in mouse embryonic stem cells: the lacZ reporter is activated by splicing, reflects endogenous gene expression, and is mutagenic in mice. Genes Dev. 6: 903-918, 1992. PubMed: 1592261 Gossler A, et al. Mouse embryonic stem cells and reporter constructs to detect developmentally regulated genes. Science 244: 463-465, 1989. PubMed: 2497519 Alexandra L Joyner, personal communication |
| Shipped： | frozen |