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| 产品名称： | pJAK14 JK388 |
|---|---|
| 商品货号： | TS142269 |
| Designations： | pJAK14 JK388 |
| Depositors： | JA Kornacki |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli SCS1 |
| Vector Information： | Size (kb): 11.0000000000000000 DESCRIPTION OF VECTOR: Intact vector size: 11.000 Type of vector: plasmid Cloning sites: EcoRI SacI Ecl136II KpnI BamHI XbaI HindIII Polylinker sites: EcoRI SacI Ecl136II KpnI XmaI SmaI BamHI XbaI AccI SalI HincII PstI SphI HindIII Construction: pMMB67EH (ATCC 37622), Tn5 Host range: broad host range; Escherichia coli Features (with orientation and position when available): promoter: tac MCS: EcoRI...HindIII, -> transcription terminator: rrnB marker(s): kanR (neoR) replicon: oriV replicon: oriT replicon: RSF1010 repressor gene: lacIq Vector: pJAK14 (plasmid) Promoters: Promoter tac Construction: pMMB67EH (ATCC 37622), Tn5 Marker(s):kanR (neoR) Construct size (kb): 11.0 Features: marker(s): kanR (neoR) promoter: tac replicon: RSF1010 replicon: oriT replicon: oriV repressor gene: lacIq MCS: EcoRI...HindIII transcription terminator: rrnB |
| Applications： | expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--11.0; HindIII--11.0; PstI--8.2, 1.85, 1.0. A shuttle vector permitting autoregulated high-level expression in all gram-negative bacteria. Compatible with common E. coli cloning vectors, as well as vectors derived from IncP and IncW plasmids. Can be mobilized by conjugative IncP helper plasmids e.g. pRK2013 (ATCC 37159), pUB307 into Klebsiella aerogenes, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens, and other gram-negative bacteria. Belongs to a group of vectors (ATCC 37622-37623, 77287-77292) differing only in antibiotic resistance and polylinker orientation. Differs from pJAK15 (ATCC 77290) only in the orientation of the polylinker. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Furste JP, et al. Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene 48: 119-131, 1986. PubMed: 3549457 Jon A Kornacki, personal communication |
| Related Products： | component of:ATCC 87055 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |