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| 产品名称： | pGEM-7Zf(+)/LIC-F |
|---|---|
| 商品货号： | TS142337 |
| Designations： | pGEM-7Zf(+)/LIC-F |
| Depositors： | RS Haun |
| Other IDs： | Nucleotide (GenBank) : U25267 Ligation-independent cloning vector pBluescript II KS(+)/LIC, complete sequence. |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.0329999923706060 Vector: pGEM-7Zf(+)/LIC-F (phagemid) Promoters: Promoter T7 Construction: pGEM-7zf(+) Marker(s):ampR Construct size (kb): 3.032999992370606 Features: insert detection: lacZ marker(s): ampR promoter: SP6 promoter: T7 promoter: lac replicon: f1 replicon: pMB1 MCS: ApaI...NsiI |
| Applications： | vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): SmaI--2.95; EcoRI--2.95; BamHI--2.95. Preparation of the vector for cloning includes linearization with NarI, gel purification of the linearized vector, and treatment with T4 DNA polymerase in the presence of dATP. The target sequence can be amplified using sequence specific primers modified at the 5 end to contain an additional 13 nt complementary to the vector sequence. The forward primer should contain 5-CTGGTTCCGGCGA-3 followed by 12-15 nt target specific sequence. The reverse primer should contain 5-CTCGCTCCGGCGA-3 followed by 12-15 nt target specific sequence. Following amplification, the amplified sequence should also be gel purified and treated with T4 DNA polymerase in the presence of dTTP. Annealing of the vector and amplification product forms a duplex molecule that can be used directly for transformation. Sequences amplified using these primers are also compatible with the pBluescript II KS(+)/LIC and pGEM-7Zf(+)/LIC-R vectors (ATCC 87047 and 87049). Differs from pGEM-7Zf(+)/LIC-R (ATCC 87049) only in the orientation of complementary ends generated at the cloning site. Ligation-independent cloning vector. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Haun RS, et al. Rapid, reliable ligation-independent cloning of PCR products using modified plasmid vectors. BioTechniques 13: 515-518, 1992. PubMed: 1362067 |
| Shipped： | freeze-dried |