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| 产品名称： | pHP45omegavph |
|---|---|
| 商品货号： | TS142453 |
| Designations： | pHP45omegavph |
| Depositors： | J Pernodet, M Blondelet-Rouault |
| Biosafety Level： | 1 |
| Vector Information： | Construct size (kb): 4.916999816894531 |
| Applications： | integrating vector shuttle vector vector permitting positive selection for integration vector useful for interposon mutagenesis |
| Comments： | Restriction digests of the clone give the following sizes (kb): ClaI--5.0; EcoRI--2.6, 2.3; SphI--3.3, 0.9, 0.8. One of three vectors (TS142453 - 87628) containing antibiotic resistance gene cassettes, derived from the omega interposon. The cassettes can be used for insertional mutagenesis or for vector construction. Antibiotic concentration used for selection on standard media, e.g. Nutrient Agar for E. coli and R2YE for Streptomyces lividans is 30 ug/ml viomycin (Vm). After transformation of competent cells, 3 to 4 hours without selection were required for expression of the cassette, before the cells could be plated on selective medium. After transformation of comopetent cells, 3 to 4 hours without selection were required for expression of the cassette, before the cells could be plated on selective medium. It is sometimes easier to select transformed E. coli using another selection marker, and to subsequently select the ones having received omega-vph by replica plating on medium with viomycin. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Blondelet-Rouault MH, et al. Antibiotic resistance gene cassettes derived from the omega interposon for use in E. coli and Streptomyces. Gene 190: 315-317, 1997. PubMed: 9197550 Jean-Luc Pernodet, personal communication |
| Shipped： | freeze-dried |