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| 产品名称： | pCF1EF+C33f D1210 (pCF1EF+C33f) |
|---|---|
| 商品货号： | TS142462 |
| Designations： | pCF1EF+C33f D1210 (pCF1EF+C33f) |
| Species： | Hepatitis C virus |
| Depositors： | Chiron Corp., L Chung, Chiron Corp. |
| Applications： | expresses antigenic determinant nonstructural protein 5 |
| Vector： | Construct size (kb): 3.5 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.300000011920929 Gene product: nonstructural protein 5 NS5 Target Gene: nonstructural protein 5 |
| Insert Size (kb)： | 0.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Expression of the SOD/HCV fusion protein is inducible by IPTG. The hepatitis C virus (HCV) insert encodes amino acids 2287 - 2385 of the HCV polyprotein. The pSODCF1 vector was modified by removing the small BamHI/EcoRI fragment (thus losing the internal SmaI site), and then inserting a linker that restored the BamHI and EcoRI sites in the appropriate reading frame, and introduced a downstream AflII site. |
| References： | Houghton M, et al. HCV immunoassays employing C domain antigens. US Patent 5,350,671 dated Sep 27 1994 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |