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| 产品名称： | pGBM6 |
|---|---|
| 商品货号： | TS142502 |
| Designations： | pGBM6 |
| Depositors： | J Geiselmann |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.4279999732971190 Vector: pGBM6 (plasmid) Promoters: Promoter lac Construction: pSC101, pUC18 MCS Marker(s):spcR,strR Construct size (kb): 4.427999973297119 Features: insert detection: lacZ marker(s): strR/spcR promoter: lac replicon: pSC101 MCS: HindIII...EcoRI |
| Applications： | The vector can be used whenever it is necessary to maintain two plasmids within the same cell. The vector expresses the alpha amino-terminal fragment of the lacZ gene product and display alpha-complementation in appropriate hosts. |
| Comments： | Restriction digests of the clone give the following sizes (kb): PvuI--3.4, 1.05; BglI-- 2.4, 1.1, 0.9; HindIII-- 4.4. The vector can be used whenever it is necessary to maintain two plasmids within the same cell. The vector expresses the alpha amino-terminal fragment of the lacZ gene product and display alpha-complementation in appropriate hosts. The vector was constructed by inserting 241/678 fragment of pUC18 into pGBM1 (ATCC 87497). |
| Media： | ATCC® Medium 1639: LB medium (ATCC medium 1065) with 50 mcg/ml spectinomycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Manen D, et al. A sensitive reporter gene system using bacterial luciferase based on a series of plasmid cloning vectors compatible with derivatives of pBR322. Gene 186: 197-200, 1997. PubMed: 9074496 |
| Shipped： | freeze-dried |