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| 产品名称： | prbTRH479 TPH479 |
|---|---|
| 商品货号： | TS142517 |
| Designations： | prbTRH479 TPH479 |
| GenBank Number： | M17250 |
| Species： | Oryctolagus cuniculus, rabbit |
| Depositors： | SL Woo |
| Vector： | Construct size (kb): 11.14000034332275 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.440999984741211 Tissue: pineal body Gene product: tryptophan hydroxylase (tryptophan 5-monooxygenase) TPH |
| Insert Size (kb)： | 2.441 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The following restriction enzymes do not cut the insert: AatII, BalI, BamHI, BglI, BglII, BstEII, BstXI, HpaI, KpnI, MluI, MstI, NaeI, NarI, NcoI, NdeI, NruI, PaeR7I, PstI, PvuI, SalI, SfiI, SmaI, XbaI, and XhoI. Restriction sites (with nucleotide positions relative to the published sequence) include: AvaII--476; BclI--1548; EcoRI--624; EcoRV--747; HincII--323; HindIII--1262; PvuII--770, 1454; SacI--673, 1833; XmnI--240. Restriction digests of the clone give the following sizes (kb): EcoRI--8.6, 1.9, 0.6; HindIII--6.3, 1.7, 1.3, 1.2, 0.5; BamHI--6.5, 3.2, 1.0; consistent with deposited DNA. The insert contains an EcoRI site at nucleotide 624 so that an EcoRI digest yields 2 fragments from the insert. Contains the complete coding sequence (nt 132-1464). |
| References： | Grenett HE, et al. Full-length cDNA for rabbit tryptophan hydroxylase: functional domains and evolution of aromatic amino acid hydroxylases. Proc. Natl. Acad. Sci. USA 84: 5530-5534, 1987. PubMed: 3475690 Savio L Woo, personal communication |