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| 产品名称： | pJS97 |
|---|---|
| 商品货号： | TS142575 |
| Designations： | pJS97 |
| Depositors： | P Hieter |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.3000001907348630 Vector: pJS97 (YAC) Promoters: Promoter T7 (phi10) Marker(s):URA3,ampR,SUP11 Construct size (kb): 7.300000190734863 Features: marker(s): ampR, URA3, SUP11 promoter: T7 (phi10) replicon: pMB1, ARSH4 centromere: CEN4 |
| Applications： | YL-type (YAC) shuttle vector shuttle vector vector for constructing genomic libraries vector for other uses vector permitting RNA synthesis in vitro |
| Comments： | Restriction digests of the clone give the following sizes (kb): NotI--7.4; BglII--7.4; SalI--7.4; EcoRI--7.4; SacI--7.4. pJS97 (TS142575) and pJS98 (ATCC 77154) are used together to construct artificial chromosomes. Each plasmid is digested with ClaI and the cloning enzyme. To recover both ends of a YAC insert as plasmid, DNA of a YAC-containing strain is digested to completion with BamHI, SphI or SacI, diluted, ligated, and transformed into E. coli (to ampicillin resistance). The insert of a resultant plasmid (which includes the T7 promoter) extends from an end of the YAC insert to the first site of the enzyme used for digestion. YL-type shuttle vector useful for construction of genomic libraries. Contains promoters for in vitro RNA synthesis and permits recovery of end fragments as plasmids in E. coli. The order of the major features in this plasmid is: ClaI/MCS/EcoRI - T7 promoter - ARSH4 - CEN4 - URA3 - SUP11 - pMB1 ori - bla - SacI - BamHI - SphI - TEL. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Shero JH, et al. Yeast artificial chromosome vectors for efficient clone manipulation and mapping. Genomics 10: 505-508, 1991. PubMed: 2071158 |
| Related Products： | component of:ATCC 77191 |
| Shipped： | freeze-dried |