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| 产品名称： | pPW121 |
|---|---|
| 商品货号： | TS142605 |
| Designations： | pPW121 |
| Depositors： | F Puta |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.3860001564025880 Vector: pPW121 (plasmid) Promoters: Promoter lambda PR Construction: pUN121 Marker(s):ampR,tetR Construct size (kb): 4.386000156402588 Features: marker(s): ampR marker(s): tetR operator: lac promoter: lambda PR replicon: pMB1 repressor gene: cI |
| Applications： | vector permitting positive selection for inserts |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; HindIII--4.3; BamHI--4.3. Postive selection cloning vector which confers tetR (recommended concentration 10 ug/ml) only when an insert has been successfully cloned into the cI repressor gene. Plasmid was derived from pUN121 by introduction of a BglII site into the cI gene. Plasmid pUN121 was derived from pTR262 by addition of EcoRI and SmaI/XmaI sites in the cI gene. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Puta F, Wambutt R. Construction of a new Escherichia coli-Saccharomyces cerevisiae shuttle plasmid cloning vector allowing positive selection for cloned fragments. Folia Microbiol. 37: 193-198, 1992. PubMed: 1505881 Nilsson B, et al. An improved positive selection plasmid vector constructed by oligonucleotide mediated mutagenesis. Nucleic Acids Res. 11: 8019-8030, 1983. PubMed: 6316281 Frantisek Puta, personal communication |
| Shipped： | freeze-dried |