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微信小章| 产品名称： | pLRbRNL |
|---|---|
| 商品货号： | TS142652 |
| Designations： | pLRbRNL |
| GenBank Number： | M28419 |
| Species： | Homo sapiens, human |
| Depositors： | W Lee |
| Applications： | in another host, produces protein retinoblastoma 1 (including osteosarcoma) |
| Vector： | Construct size (kb): 10.39999961853027 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.700000047683716 Gene product: retinoblastoma 1 (including osteosarcoma) RB1 Alleles: A1, A2, A1, A2 Target Gene: retinoblastoma 1 (including osteosarcoma) |
| Insert Size (kb)： | 3.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert contains an EcoRI site (1028) and a BglII site (1970). Restriction digests of the clone give the following sizes (kb): HindIII/ClaI--4.3 (doublet), 1.8; EcoRI--4.5, 3.5, 1.1, 0.9; HindIII--6.0, 4.3; BamHI--6.5, 4.0; PstI--3.8, 2.8, 2.0, 1.9. The O.9 kb EcoRI/BglII fragment detects genomic HindIII fragments of the following sizes (kb): 7.0, 1.3, 5.5, 4.5, 7.5, 5.3. The insert consists of the first 3686 nucleotides of the published sequence, including the complete coding sequence (138 - 2922) plus 5 untranslated and partial 3 untranslated regions. The construct expresses a normal-sized protein product in transfected mouse and rat cells. This plasmid was constructed from a 4.3 kb HindIII/ClaI fragment containing 2 MoMLV LTR sequences flanking a beta-lactamase gene, a 2.4 kb SmaI/ClaI fragment containing RSV-promoter-Neo, and the 3.7 kb HindIII/ScaI cDNA fragment. This sequence in the insert was constructed from cDNAs cloned from several sources. |
| References： | Huang HJ, et al. Suppression of the neoplastic phenotype by replacement of the RB gene in human cancer cells. Science 242: 1563-1566, 1988. PubMed: 3201247 Lee WH, et al. Human retinoblastoma susceptibility gene: cloning, identification, and sequence. Science 235: 1394-1399, 1987. PubMed: 3823889 Lee WH, et al. The retinoblastoma susceptibility gene encodes a nuclear phosphoprotein associated with DNA binding activity. Nature 329: 642-645, 1987. PubMed: 3657987 |