微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pJ3H |
|---|---|
| 商品货号： | TS142693 |
| Designations： | pJ3H |
| Depositors： | J Chernoff |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.5000000000000000 Vector: pJ3H (plasmid) Promoters: Promoter SV40 Early Construction: pJ3omega Marker(s):ampR Construct size (kb): 3.5 Features: marker(s): ampR other: SV40 t IVS promoter: SV40 Early replicon: SV40 replicon: pMB1 MCS: XbaI...BglII terminator: SV40 T polyadenylation epitope tag: hemagglutinin (HA) |
| Applications： | encodes an epitope tag for protein isolation or monitoring expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.5; EcoRI--3.5; PvuI--1.95, 1.5. Shuttle expression vector encoding an influenza hemagglutinin (HA) epitope tag, recognized by monoclonal antibody 12CA5, to facilitate protein isolation and monitoring. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Sells MA, Chernoff J. Epitope-tag vectors for eukaryotic protein production. Gene 152: 187-189, 1995. PubMed: 7530674 |
| Shipped： | freeze-dried |