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| 产品名称： | pPC1A |
|---|---|
| 商品货号： | TS142774 |
| Designations： | pPC1A |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | IK Yu |
| Applications： | in another host, produces protein |
| Insert： | DNA: cDNA Insert lengths(kb): 2.799999952316284 Gene product: pPC1A |
| Insert Size (kb)： | 2.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: frozen |
| Comments： | A gene encoding an endocellulase from Clostridium strain IY-2 which can digest bamboo fibers, cellulose, rice straw, and sawdust, was isolated by shotgun cloning in an E.coli expression plasmid pLC2833. E.coli k-12 comprising a vector, pLC2833 which contains a promoter and a 2.8 kb Clostridium strain IY-2 DNA insert which is further cleaved in to 2.2 kb DNA fragment comprising at least an entire structural gene and signal sequences of cellulase. |
| Classification： | Enterobacteriaceae, Escherichia |
| References： | Remaut E, et al. Plasmid vectors for high-efficiency expression controlled by the PL promoter of coliphage lambda. Gene 15: 81-93, 1981. PubMed: 6271633 Yu IK. Secretion of Clostridium cellulase by E. coli. US Patent 5,496,725 dated Mar 5 1996 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |