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| 产品名称： | pCN51 |
|---|---|
| 商品货号： | TS142791 |
| Designations： | pCN51 |
| Depositors： | R Diaz Orejas |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.9000000953674320 Vector: pCN51 (plasmid) Construction: pCN50 Marker(s):kanR Construct size (kb): 5.900000095367432 Features: marker(s): kanR replicon: repA, pMB1 |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--4.4, 1.4; EcoRI--4.8, 1.0; PvuII--5.8. Contains the basic replicon of pPS10 which has been sequenced (EMBL accession number X58896). The repA portion is flanked by EcoRI sites (nt 536 and 1500), with the site at nt 536 between the repA promoter and coding sequences. Retains the mobilization site of pBR322-derived vectors and can be mobilized if suitable functions are provided in trans. Shuttle vector. A spontaneous deletion derivative of pCN50 (77099). The order of the major features in this plasmid is: PvuII - pMB1 ori - BamHI - kanR - HindIII - repA. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Nieto C, et al. Cloning vectors, derived from a naturally occurring plasmid of Pseudomonas savastanoi, specifically tailored for genetic manipulations in Pseudomonas. Gene 87: 145-149, 1990. PubMed: 2110095 Ramon Diaz Orejas, personal communication |
| Shipped： | frozen |
| Shipping Information： | Frozen glycerol stock of xa0E. coli containing the plasmid. |