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| 产品名称： | M31A |
|---|---|
| 商品货号： | TS142820 |
| Designations： | M31A |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 51.70000076293945 |
| Insert： | DNA: genomic Insert lengths(kb): 40.0 Gene product: tumor necrosis factor, beta (lymphotoxin)(tumor necrosis factor, beta, lymphotoxin) TNFA |
| Insert Size (kb)： | 40.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--18.0, 9.6, 7.8, 4.8, 2.0, 1.7, 1.4, 0.7; KpnI--32.0, 11.4, 6.7, 1.6; SalI--38.3, 8.4, 5.1; PstI--5.2, 5.1, 3.5, 3.5, 3.4, 3.2, 3.0, 2.8, 2.8, 2.3, 2.1, 2.0, 1.6, 1.5, 1.4, 1.4, 1.1, 0.9, 0.7, 0.7, 0.65, 0.65. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Cosmid contains coding sequences for both TNFalpha and TNFbeta; fragment containing both can be excised by KpnI/BamHI digest (approx. 7 kb). |
| References： | Spies T, et al. Genes for the tumor necrosis factors alpha and beta are linked to the human major histocompatibility complex. Proc. Natl. Acad. Sci. USA 83: 8699-8702, 1986. PubMed: 3464978 Taylor MF, et al. In vitro efficacy of morpholino-modified antisense oligomers directed against tumor necrosis factor-alpha mRNA. J. Biol. Chem. 271: 17445-17452, 1996. PubMed: 8663413 |