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微信小章| 产品名称： | pMLC3/4 |
|---|---|
| 商品货号： | TS142905 |
| Designations： | pMLC3/4 |
| GenBank Number： | K02782 |
| Species： | Mus musculus, mouse |
| Depositors： | GH Fey |
| Vector： | Construct size (kb): 6.699999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.400000095367432 Tissue: liver, adult male Gene product: complement component 3 C3 |
| Insert Size (kb)： | 2.400 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: frozen |
| Comments： | The insert includes the following restriction sites (nucleotide positions according to GenBank K02782): PstI--2676, 3511, 3607, 4846; PvuI--4017; StuI--4041; SalI--4677; HindIII--5060. Restriction digests of the clone give the following sizes (kb): EcoRI--6.7; SalI--4.9, 1.8; PstI--4.3, 1.3, 0.8, 0.3; HindIII--5.9, 0.8. pMLC3/4 contains the 3 portion of the cDNA, encoding most of the alpha chain portion which begins at nucleotide 2067. The 5 end of the insert is between the StuI (2223) and PstI (2676) sites. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Domdey H, et al. Characterization of the mRNA and cloned cDNA specifying the third component of mouse complement. Proc. Natl. Acad. Sci. USA 79: 7619-7623, 1982. PubMed: 6961437 Wetsel RA, et al. Structure of murine complement component C3. II. Nucleotide sequence of cloned complementary DNA coding for the alpha chain. J. Biol. Chem. 259: 13857-13862, 1984. PubMed: 6094532 Fey GH, et al. Nucleotide sequence of complementary DNA and derived amino acid sequence of murine complement protein C3. Philos. Trans. R. Soc. Lond. B Biol. Sci. 306: 333-344, 1984. PubMed: 6208565 |