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| 产品名称： | pMSEx-2 |
|---|---|
| 商品货号： | TS142941 |
| Designations： | pMSEx-2 |
| Depositors： | M Schuermann |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.5999999046325680 Vector: pMSEx-2 (plasmid) Promoters: Promoter SV40 early Construction: pMSE (ATCC 37834) Marker(s):G418R,ampR,kanR Construct size (kb): 5.599999904632568 Features: marker(s): ampR, kanR, G418R promoter: SV40 early replicon: pMB1 terminator: MSV LTR enhancer: SV40 early |
| Applications： | expression vector integrating vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.6, 1.7, 1.0, 0.3; HindIII--2.9, 2.7; EcoRI--4.2, 1.4; BglII--5.6. A EcoRI/HindIII digest gives fragments of 2.63, 1.68, 0.97 and 0.32 kb. The SV40 promoter has a 3 bp deletion at the BglI site within the origin of replication to prevent autonomous replication in the presence of SV40 large T-antigen. The sequence of the oligonucleotide is AGCTTGGTAC CATGGACCTC GAGGCCTAGC TAGCTAG GAGCT. Kanamycin resistance should be checked at regular intervals. This is best done during amplification steps using LB medium supplemented with 50 ug/ml ampicillin and 12.5 ug/ml kanamycin. One of 3 shuttle expression vectors (pMSEx-1-3, ATCC 37835-37837) containing a synthetic oligonucleotide providing a translation initiation signal, followed by a StuI site in 3 different positions, followed by a stop signal in all reading frames. The MSV LTR provides a polyadenylation signal as well as an enhancer for the P1/thymidine kinase promoter regulating the Tn5 kanR/G418R transcription unit. The order of the major features in this plasmid is: SV40 promoter - oligonucleotide/StuI/oligonucleotide - MLV-LTR - P1 promoter - TK promoter - kanR/G418R - TK polyadenylation signal - ampR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Schuermann M. An expression vector system for stable expression of oncogenes. Nucleic Acids Res. 18: 4945-4946, 1990. PubMed: 2395667 Marcus Schuermann, personal communication |
| Shipped： | freeze-dried |