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微信小章| 产品名称： | Cryptococcus gattii (Vanbreuseghem et Takashio) Kwon-Chung et Boekhout |
|---|---|
| 商品货号： | TS142958 |
| Deposited As： | Cryptococcus bacillisporus |
| Strain Designations： | A1M R265 CBS 10514 |
| Alternate State： | Filobasidiella bacillispora Kwon-Chung, teleomorph |
| Application： | Multigene phylogeny and phenotypic characterization. Biomedical Research and Development Material |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Antigenic Properties： | Serotype B |
| Genotype： | VGIIa, AFLP6A |
| Preceptrol®： | no |
| Genome Sequenced Strain： | Yes |
| Mating Type： | alpha |
| Comments： | Genome sequencing strain (Broad Institute, USA)
Cause cryptococcosis
Represents one of the two genotypes causing an outbreak in Vancouver Island
Multigene phylogeny and phenotypic characterization
Designated as type strain of Cryptococcus deuterogattii Hagen & Boekhout by Hagen et al. (2015) |
| Medium： | ATCC® Medium 28: Emmons modification of Sabourauds agar ATCC® Medium 200: YM agar or YM broth ATCC® Medium 1245: YEPD |
| Growth Conditions： | Temperature: 24°C to 26°C Atmosphere: Typical aerobic |
| Sequenced Data： |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence GGTTTCCGTAGGTGAACCTGCGGAAGGATCAGTAGAGAATACTGGACTTCGGTCCATTTATCTACCCATCTACACCTGTGAACTGTTTATGTGCTTCGGCACGTTTTACACAAACTTCTAAATGTAATGAATGTAATCTTATTATAACAATAATAAAACTTTCAACAACGGATCTCTTGGCTTCCACATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCAACTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGAGTCATGAAAATCTCAATCCCTCAGGTTTTATTACCTGTTGGACTTGGATTTGGGTGTTTGCCGCGACCTGCAAAGGACGTCGGCTCGCCTTAAATGTGTTAGTGGGAAGGTGATTACCTGTCAGCCCGGCGTAATAAGTTTCGCTGGGCCTATGGGGTAGTCTTCGGCTTGCTGATAACAACCATCTCTTTTTGTTTGACCTCAAATCAGGTAGGGCTACCCGCTGAACTTAAGCATATCAATAA D1D2 region of the 26S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGTGAACCGGGAAGAGCTCAAATTTGAAATCTGGCGTCCTCCGGGCGTCCGAGTTGTAATCTACAGAAACGTTTTCCGTGCTGGACCGTGTCTAAGTCCCTTGGAATAGGGTATCAAAGAGGGTGACAATCCCGTACTTGACACGATCACCAGTGCTCTGTGATACGTTTTCTACGAGTCGCGTTACTTGGGAGTGTAGCGCAAAATGGGTGGTAAACTCCATCTAAAGCTAAATATTGGTGGAAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAACGATTGAAGTCAGTCGTGTCTATTGGGTTCAGCCAGTTCTGCTGGTGTATTCCCTTTAGACGGGTCAACATCAGTTCTGATCGGTGGATAAGGGCTGGAGGAATGTGGCACTCTTCGGGGTGTGTTATAGCCTCCTGTCGCATACACTGGTTGGGACTGAGGAATGCAGCTCGCCTTTATGGCCGGGGTTCGCCCACGTTCGAGCTTAGGATGTTGACAAAATGGCTTTAAACGAC 26S ribosomal RNA gene and intergenic spacer (IGS), partial sequence ATGGGAACGGGGTGCTGTAAGTGGTAGAGTAGCCTTGTTGCTACGATCCACTGAGGCTAAGCCCTTGTTCTATAGATTTGTCTCTAATATGTTAGGTCTCGGGGGGCTTCCTCTAGAGACTTGGATGTAAGAGGCTTTGCTTTTTTTTATGACGATGACTTGTCAAAGTGTGGATGACTCCAGGAGAGAGGTCGGGCTGGTAATTAGTAATATCAGTCAGTCATTTCAGCTGGCGCCATCGATACTTTATAAGTCAAACTTAACCATTTCACTGTGTAGTAAGTAGTAGGCTCTGAATTACTAGAGACACTTGCCAGGTTATCGCAAGTTGGCAGGCAGGCAGGACACACATACTATTGATTTCACGGTCATGGGGGACTTGGCTGCTGGTGCTTGAGTTGCATAATAGAGAGAGTATATGCATGTCGCGGGGGGGGGACTTGGCTAAGATGCGTTATGCGGCAAGCAAGGTAAGTAGTATTTGTATTAAAGTATTTAGTTACTTTTCCAGTCATGGGGGACTTTGATAGTGGTTGTGAGCCGGAATGAAAAATGGGTAAATGTGGTATGGATGGTGAGAGGGGGAGCTGCCAAGTGTATGCAGTGGGTGTGCTTATAGCATAGTCAAGAGGGGATGGGCAATTAAATTTTGGAAAGTTGTTTGACCCGACCTGACGGTGACTCTAATAGGCTGGTCAAGCAAACGTTTAAGTTGAGTCAAGCTCGGTCAAGCAAAGTCTAGAAAAGATTAATGATCGCTAAGAATTACTCCGGTCGCGGGGGGCTTGCAACTTGTCTGGCCCACTGTGGGTCCAGTTT glycerol-3-phosphate dehydrogenase 1 (GPD1) gene, partial cds TACTTGCATAAATACAATGGTTGTCAAGGTTGGAATCAACGGTTTCGGTACGTGATCTGTCTCTATCAAGCGTTGCTTCATATGTCACCAATGTGGTTTCCGATATTGTGCACCGTCCTTGGGTTACTGGTAGCTAATAGTTATGCGATAGGTCGTATCGGTCGAATTGTTCTCAGGTATGCTTTAAGTCAAGGGGTAAGACAGCTGCGTATCTAACCATCAAATTAGGAACGCCATCGAGCATGGAGACCTTGAGGTTGTTGCTGTCAACGAGTGAGTATTCTCAAAAGTGTTAATCTATTAAATAAATTATTTACTTTTGCAAAGCCCTTTCATTGACTTGGACTACATGGTATAATACTTTCTTCTGTACAATTGCTTCGTTTTGTGCTAACTGTAGCATGCGTAGGTTTACATGTTCAAGTACGACTCCGTAAGTCTCTGATGGCTCTAGCGCTCTATATGGCATTTTAACCTCATTCCCAACAGACACATGGTCGCTTCAAGGGTTCTGTCGAGGTTAAGGACGGTAAGCTCTATATCAACAACAAGGCCATTTCCGTTTTCGGTGAGAAGGACCCCGCCAACATCAAGTGGGGTGACGCTGGTGCCGAGTACATCGTTGAGTCTACCGGTGTCTTCACCACTACCGAAAAGGCCGGTGTCCAT |
| Name of Depositor： | L Hoang |
| Special Collection： | NSF - Mycology |
| Chain of Custody： | ATCC |
| Isolation： | Bronchial alveolar lavage, Duncan, Vancouver Island, B.C. Canada. |
| Cross References： | Nucleotide (GenBank) : AAFP00000000 Cryptococcus gattii VGII R265, whole genome shotgun sequencing project |
| References： | Hu G, Kronstad JW. Gene disruption in Cryptococcus neoformans and Cryptococcus gattii by in vitro transposition. Curr Genet 49: 341-350, 2006. PubMed: 16397763 Fraser James A, et al. Same-sex mating and the origin of the Vancouver Island Cryptococcus gattii outbreak. Nature 437: 1360-1364, 2005. Kidd Sarah E, et al. Comparative gene genealogies indicate that two clonal lineages of Cryptococcus gattii in British Columbia resemble strains from other geographical areas. Eukaryot. Cell 4: 1629-1638, 2005. Kidd Sarah E, et al. A rare genotype of Cryptococcus gattii caused the cryptococcosis outbreak on Vancouver Island (British Columbia, Canada). Proc. Natl. Acad. Sci. USA 101: 17258-17263, 2004. Findley K, et al. Phylogeny and phenotypic characterization of pathogenic Cryptococcus species and closely related saprobic taxa in the Tremellales. Eukaryot Cell 8(3): 353-361, 2009. PubMed: 19151324 Wang H, et al. A fungal phylogeny based on 82 complete genomes using the composition vector method. BMC Evol. Biol. 9: 195, 2009. PubMed: 19664262 Meyer W. et al. Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii. Med. Mycol. 47(6):561-570, 2009. PubMed: 19462334 DSouza CA, et al. Genome variation in Cryptococcus gattii, an emerging pathogen of immunocompetent hosts. mBio 2: e00342-10, 2011. PubMed: 21304167 Hagen F, et al. Recognition of seven species in the Cryptococcus gattii/Cryptococcus neoformans species complex. Fungal Genet. Biol. 78: 16-48, 2015. PubMed: 25721988 |