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| 产品名称： | Hf934 Hf-934 |
|---|---|
| 商品货号： | TS142983 |
| Designations： | Hf934 Hf-934 |
| Species： | Homo sapiens, human |
| Depositors： | M Chu |
| Vector： | Construct size (kb): 5.699999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.299999952316284 Tissue: fibroblast Gene product: collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant) COL3A1 |
| Insert Size (kb)： | 1.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--4.4, 0.7, 0.6; BamHI--5.7; PstI--4.1, 1.6; EcoRI--5.1, 0.6; SalI--5.7. The insert includes sequence beginning about 0.8 kb upstream of the stop codon and a 3 noncoding sequence of 223 bp and poly(A). This probe detects mRNA of 5.4 and 4.8 kb in human fibroblasts. |
| References： | Chu ML, et al. Isolation of cDNA and genomic clones encoding human pro-alpha1(III) collagen. Partial characterization of the 3 end region of the gene. J. Biol. Chem. 260: 4357-4363, 1985. PubMed: 2579949 Huerre-Jeanpierre C, et al. Further evidence for the dispersion of the human fibrillar collagen genes. Am. J. Hum. Genet. 38: 26-37, 1986. PubMed: 3004202 Mon-Li Chu, personal communication |