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微信小章| 产品名称： | 23 ScCr 10ScNCr/23 |
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| 商品货号： | TS143070 |
| Organism： | Mus musculus, mouse |
| Tissue： | bone marrow |
| Cell Type： | macrophage |
| Product Format： | frozen |
| Morphology： | macrophage |
| Culture Properties： | mixed, adherent and suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | FIV |
| Age： | 6 months adult |
| Strain： | C57BL/10ScN (formerly C57BL/10ScNCr, C57BL/10ScCr) |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | 23 ScCr is a bone marrow derived macrophage cell line developed from a mouse strain with a deletion of the TLR4 locus. |
| Antigen Expression： | CD11a, mouse; Mus musculus CD11b, mouse; Mus musculus F4/80, mouse |
| Genes Expressed： | Macrophage inflammatory protein-2 tumor necrosis factor alpha (TNF alpha) |
| Cellular Products： | Macrophage inflammatory protein-2 tumor necrosis factor alpha (TNF alpha) |
| Comments： | The cell line is deficient in toll-like receptor 4 (TLR4). Stimulation with lipoteichoic acid (LTA) induces a five to six fold increase in tumor necrosis factor alpha (TNF alpha) and macrophage inflammatory protein-2 (MIP-2) secretion. No increases in either cytokine were found when cells were stimulated with lipopolysaccharides (LPS). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 70%; fetal bovine serum, 10%; LADMAC Conditioned Media (produced from the LADMAC cell line (CRL-2420), 20%
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| Subculturing： | Remove 75% of the media. Scrape off the attached cells. Resuspend cells at 2xa0x 105 viable cells/mL. Transfer into new flasks.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
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| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | E Lorenz |
| Deposited As： | Mus musculus |
| References： | Lorenz E, et al. Toll-like receptor 4 (TLR4)-deficient murine macrophage cell line as an in vitro assay system to show TLR4-independent signaling of Bacteroides fragilis lipopolysaccharide. Infect. Immun. 70: 4892-4896, 2002. PubMed: 12183533 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |